TAJ is a member of the TNF receptor superfamily. This receptor is highly expressed during embryonic development. It has been shown to interact with TRAF family members, and to activate the JNK signaling pathway when overexpressed in cells. This receptor is capable of inducing apoptosis by a caspase-independent mechanism, and it is thought to play an essential role in embryonic development. Alternatively spliced transcript variants encoding distinct isoforms have been described.
Suitable for use in Flow Cytometry. Other applications not tested.
Flow Cytometry: 1ug/ml
Optimal dilutions to be determined by the researcher.
Flow Cytometry Protocol:
1. 5x10e5 transfected HEK 293T cells were incubated on ice for 30 min in 50ul FACS buffer (PBS, 5% Fetal calf serum, 0.02% azide) containing 1ug/ml of T0721-06.
2. After washing in FACS buffer, R-PE-conjugated antibody to rat IgG (I1904-74D) was added.
3. Cells were incubated on ice for 30 min, washed and analyzed by flow cytometry.
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.