T-box expressed in T cells (T-bet), also known as T-box transcription factor TBX21, is a 62kD member of the T-box family of transcription factors and the Tbr1 subfamily. T-bet is a nuclear protein that is Th1-cell specific. Human T-bet is 535aa in length and contains a T-box DNA binding domain (aa136-327; SwissProt Accession # Q9UL17). Human T-bet shares 88% aa
sequence identity with mouse T-bet. Northern blot analysis revealed that it is expressed only in the lung, thymus and spleen. Functionally, T-bet controls the expression of the Th1 cytokine, IFN-gamma, and initiates Th1 lineage development from nai?ve Th precursor cells by both activating Th1 genetic programs and by repressing the opposing Th2 programs.
Suitable for use in Flow Cytometry. Other applications not tested.
Optimal dilutions to be determined by the researcher.
Storage and Stability:
Lyophilized powder may be stored at 4 degrees C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.