T-box expressed in T cells (T-bet), also known as T-box transcription factor TBX21, is a 62kD member of the T-box family of transcription factors and the Tbr1 subfamily. T-bet is a nuclear protein that is Th1-cell specific. Human T-bet is 535aa in length and contains a T-box DNA binding domain (aa136-327; SwissProt Accession # Q9UL17). Human T-bet shares 88% aa
sequence identity with mouse T-bet. Northern blot analysis revealed that it is expressed only in the lung, thymus and spleen. Functionally, T-bet controls the expression of the Th1 cytokine, IFN-gamma, and initiates Th1 lineage development from nai?ve Th precursor cells by both activating Th1 genetic programs and by repressing the opposing Th2 programs.
Applications:
Suitable for use in Flow Cytometry. Other applications not tested.
Recommended Dilution:
Optimal dilutions to be determined by the researcher.
Storage and Stability:
Lyophilized powder may be stored at 4 degrees C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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