The syntrophins are a family of scaffolding proteins with multiple protein interaction domains that link signaling proteins to dystrophin family members. Each of the three most characterized syntrophins (alpha, beta-1, and beta-2) contains a PDZ domain that binds a unique set of signaling proteins including kinases, ion and water channels, and neuronal nitric oxide synthase (nNOS). Despite their ability to bind dystrophin in vitro, neither g-syntrophin isoform co-localizes with dystrophin in skeletal muscle. Furthermore, g-syntrophins do not co-purify with dystrophin isolated from mouse tissue. Research suggests that the interaction of g-syntrophin with dystrophin is transient and potentially subject to regulatory mechanisms. g1-Syntrophin is highly expressed in brain and is specifically localized in hippocampal pyramidal neurons, Purkinje neurons in cerebellum, and cortical neurons. g2-Syntrophin is expressed in many tissues including skeletal muscle where it is found only in the subsynaptic space beneath the neuromuscular junction. In both neurons and muscle, g-syntrophin isoforms localize to the endoplasmic reticulum where they may form a scaffold for signaling and trafficking (Adams, 2006).
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Western Blot: 1ug/ml
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.