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Cat no: S8999-03A

Supplier: United States Biological
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Applications: Suitable for use in Flow Cytometry and Immunoprecipitation. Other applications not tested. Recommended Dilution: Flow Cytometry: Neat-1:10; 10ul labels 10e6 cells in 100ul Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. FITC conjugates are sensitive to light.
Catalogue number: S8999-03A
Reactivities: Porcine
Hosts: Mouse
Applications: Flow Cytometry, Immunoprecipitation
Size: 100ug
Form: Supplied as a liquid in PBS, pH 7.4, 1% BSA, 0.09% sodium azide. Labeled with fluorescein isothiocyanate (FITC).
P type: Mab
Isotype: IgG2a
Purity: Purified by Protein G affinity chromatography.
References: 1. Bullido, R. et al. (1999) Monoclonal antibodies 2F6/8 and 2A10/8 recognize a porcine antigen (SWC7) expressed on B cells and activated T cells. J. Immunol. Methods. 222:1-11. 2. Boersma, W.J.A.et al. (2001) Summary of workshop findings for porcine B-cell markers. Vet. Immunol. Immunopathol. 80:63-78. 3. Saalmuller, A.et al. (1998) Overview of the second international workshop to define swine cluster of differentiation (CD) antigens. Vet. Immunol. Immunopathol. 60: 207-228. 4. Denham, S.et al. (1998) Monoclonal antibodies putatively identifying porcine B cells. Vet. Immunol. Immunopathol. 60: 317-328.
Additional info: Recognizes the porcine SWC7 cell surface antigen which is expressed by activated B lymphocytes and a subset of CD3+ T lymphocytes. Immunoprecipitates a dimeric molecule with an apparent molecular size of 40kD (non-reduced) or 24kD (reduced).

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