Sulfatase Modifying Factor 2 (SUMF2) is structurally similar to SUMF1, which activates sulfatases by converting their active site residue cysteine to formylglycine. Functionally, SUMF2 does not activate sulfatases, but rather competes with SUMF1 for the same substrates. The amino acid sequence of mouse SUMF2 is 94%, 83%, 82%, 81% and 72% identical to that of rat, human, canine, chimpanzee and chicken.
Applications:
Suitable for use in Direct ELISA and Western Blot. Other applications not tested.
Recommended Dilution:
Direct ELISA: 0.5-1ug/mL detection limit for rmSUMF2 ~ 5ng/well.
Western Blot:1-2ug/mL detection limit for rmSUMF2 ~ 5ng/lane under non-reducing and reducing conditions.
Optimal dilutions to be determined by the researcher.
Storage and Stability:
Lyophilized powder may be stored at -20 degrees C. Reconstitute to nominal volume by adding sterile 40-50% glycerol, PBS. Aliquot and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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