Bacteria of the Streptococcus genera produce the exotoxin Streptolysin. Anti-Streptolysin antibody titers in patients are measured as a marker of inflammation. Streptolysin is also an important tool in cell biology due to its hemolytic activity and the ability to permeabilize plasma membranes.
Applications:
Suitable for use in ELISA. Other applications not tested.
Recommended Dilutions:
Optimal dilutions to be determined by the researcher.
Hybridoma:
X63-Ag8.653 myeloma cells with spleen cells from female CF1 x BALB/c mice.
Culture Medium:
RPMI 1640 with 10% fetal calf serum
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.