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Streptavidin

Streptavidin

Cat no: S7973-78D

Supplier: United States Biological
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Streptavidin is a tetrameric protein purified from Streptomyces sp. that binds very tightly to the vitamin biotin with a Kd of 10-14 mol/l. The high affinity recognition of biotin and biotinylated molecules has made streptavidin one of the most important components in diagnostics and laboratory kits. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:100-1:2000 detects a band at ~19kD. Immunohistochemistry (Frozen/Paraffin): 1:10-1:2000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: S7973-78D
Hosts: Mouse
Applications: Immunohistochemistry, Western Blot
Size: 500ug
Form: Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
P type: Mab
Isotype: IgG1
Purity: Purified by Protein A affinity chromatography.
Additional info: Recognizes Streptavidin. Does not cross react with egg white avidin. Binds to Streptavidin without interfering with the biotin-binding reaction.

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