Produced by hyperimmunizing goats with pure streptavidin and selecting out high affinity, specific antibody by affinity chromatography on specially prepared agarose bound streptavidin. Anti-Streptavidin and Anti-Streptavidin conjugates can be used to localize streptavidin or to amplify the signal produced by streptavidin conjugates. Biotinylated Anti-Streptavidin has the unusual property of binding to streptavidin either through its antigen binding site or through its biotin residues. This product is produced by optimally biotin-labeling our affinity-purified Anti-Streptavidin. By following a fluorochrome conjugated Streptavidin with Biotinylated Anti-Streptavidin and adding a second layer of fluorochrome conjugated Streptavidin, the fluorescent signal can be amplified. This product can be used in tissue staining, chromosome mapping, or in cell sorter analysis. In addition, Biotinylated Anti-Streptavidin is considered the industry standard for microarray applications.
Applications:
Suitable for use in ELISA. Other applications not tested.
Recommended Dilution:
Optimal dilutions to be determined by the researcher.
Storage and Stability:
Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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