STAT3 (Signal Transducer and Activator of Transcription 3, Acute-phase Response Factor, APRF) (Control Peptide)

DMELTSECATSPM from the C-terminus of the protein sequence according to NP_003141.2 and NP_644805.1. Control peptide corresponding to polyclonal antibody, Catalog#S7971-01M, goat x human. Stat3 is a key signaling molecule for many cytokines and growth-factor receptors, and is required for murine fetal development. Additionally, Stat3 is constitutively activated in a number of human tumors and possesses oncogenic potential and anti-apoptotic activities. Stat3 is activated by phosphorylation at Tyr705, which induces dimerization, nuclear translocation and DNA binding. Transcriptional activation seems to be regulated by phosphorylation at Ser727 via the MAPK or mTOR pathway. Stat3 isoform expression appears to reflect biological function: the relative expression levels of Stat3a (86kD) and Stat3b (79kD) depend on cell type, ligand exposure or maturation stage of the cells. It is notable that Stat3b lacks the serine phosphorylation site within the carboxy-terminal transcriptional activation domain. Domain Information: SH2 domain; STAT protein, DNA binding domain; STAT protein, all-alpha domain; STAT protein, protein interaction domain Applications Suitable for use in ELISA. Other applications have not been tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Peptide Blocking (see corresponding antibody S7971-01M): Antibodies are typically supplied at 0.5mg/ml and peptides as a 100ul pellet. When peptides are reconstituted in 200ul water, the concentration would be also 0.5mg/ml. To start, the best ratio would be 1:1 (which means molar excess of peptides relative to antibodies when identical volumes are mixed). Mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. It is best is to have two identical blots, to be incubated with equal amount of antibodies, but one with the antibodies pre-adsorbed to the peptide for 20min. Then incubate and develop the two blots in parallel.