Membrane receptor signaling by various ligands, including interferons and growth hormones such as EGF, induces activation of JAK kinases, which then leads to tyrosine phosphorylation of the various Stat transcription factors. Stat1 and STAT2 are induced by IFN-a and form a heterodimer, which is part of the ISGF3 transcription factor complex.
Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilution:
Immunohistochemistry (Formalin fixed paraffin embedded): 1:50 for 30min at RT
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10min followed by cooling at RT for 20min
Optimal dilutions to be determined by the researcher.
Positive Control:
Ramos cells, breast carcinoma
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
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