Home  >  Products  >  Stage Specific Embryonic Antigen 4 (SSEA4) (PE)

Stage Specific Embryonic Antigen 4 (SSEA4) (PE)

Cat no: S6610-11P

Supplier: United States Biological
0 reviews | Write a Review Pencil
Stage-Specific Embryonic Antigens (SSEAs) were originally identified by monoclonal antibodies (mAb) generated against defined carbohydrate epitopes associated with lacto- and globo-series glycolipids. The SSEA-4 specific monoclonal antibody was generated from a mouse immunized with the 2102Ep human teratocarcinoma cell line. This mAb recognizes the terminal structure of the sialyl galactosylgloboside Luke (LKE) antigen expressed on human red blood cells and platelets. SSEA-4 expression has been reported on ACHN renal tubular cells and also on normal human kidney. The expression of SSEA-4 on human teratocarcinoma-derived cell lines prompted investigations into the expression of globosides during embryonal differentiation. Embryonic carcinoma cells that express SSEA-4 rapidly lose expression of SSEA-4 as the cells differentiate). In addition, human embryonic stem cells express SSEA-4. Studies have utilized this mAb for the detection of SSEA-4 on mouse oocytes through the early blastocyst stage. However, SSEA-4 has not been detected on mouse embryonic carcinoma or mouse embryonic stem cells. Finally, studies have shown that SSEA-4 is expressed on primary sensory neurons of the rat and other species. These investigations imply a role for SSEA-4 and other cell surface carbohydrates in cellular adhesion. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 10ul labels up to 1x10e6 cells or 50ul packed whole blood. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C before opening. DO NOT FREEZE! Stable at 4 degrees C as an undiluted liquid. Dilute only prior to immediate use. Stable for 12 months at 4 degrees C. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity. PE conjugates are sensitive to light.
Catalogue number: S6610-11P
Reactivities: Human, Mouse
Hosts: Mouse
Applications: Flow Cytometry
Size: 100Tests
Form: Supplied as a liquid in saline, 0.5% BSA, 0.1% sodium azide. Labeled with R-Phycoerythrin (PE).
P type: Mab
Isotype: IgG3
Purity: Purified by Protein A affinity chromatography.
References: 1. Kannagi, R. et al. (1983) EMBO J. 2:2355. 2. Tippett, P. et al. (1986) Vox Sang. 51:53. 3. Cooling, L.L. (2001) Transfusion. 41:504. 4. Katagiri, Y.U. et al. (2001) Glycoconj. J. 18:347. 5. Karr, J.F. et al. (1990) Infect. Immunol. 58:4055. 6. Krupnick, J.G. et al. (1994) Int. J. Cancer 59:692. 7. Andrews, P.W. (1987) J. Cell. Biochem. 35:321. 8. Andrews, P.W. et al. (1996) Int. J. Cancer 66:806. 9. Carpenter, M.K.. et al. (2003) Cloning Stem Cells 5:79. 10. Dodd, J. et al. (1984) Nature 311:469.
Additional info: Recognizes human Stage Specific Embryonic Antigen 4. Species Crossreactivity: mouse

Get Quote

  • Best Price Guaranteed
  • Quick Response Time
  • Exclusive Promotions
United States Biological
Get a Quote Direct from
United States Biological

By submitting this form you agree to your details being passed to United States Biological for the purpose of generating the best quote*

Button_on Button_off_biosave Button_off_biosave Button_off_biosave Button_off_biosave Button_off_biosave Button_off_biosave