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SSEA-1 (Stage-Specific Embryonic Antigen-1, SSEA1)

Cat no: S6609-99

Supplier: United States Biological
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Stage-Specific Embyronic Antigen-1(SSEA-1), also referred to as Lewis X (LeX), is expressed on murine and human germ cells, murine embryonic stem (ES) cells and murine embryonal carcinoma (EC) cells . Expression levels differ between mouse and human with decreasing expression upon differentiation in mice and increased expression upon differentiation in humans. Applications: Suitable for use in Flow Cytometry, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested. Recommended Dilution: Flow Cytometry: 0.25ug labels10e6 cells Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: S6609-99
Reactivities: Human, Mouse
Hosts: Mouse
Applications: Flow Cytometry, Immunohistochemistry, Immunoprecipitation, Western Blot
Size: 25ug
Form: Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.
P type: Mab
Isotype: IgM
Purity: Affinity purifed
References: 1. Anjos-Afonso and Bonnet D. Blood. Feb 1;109(3):1298-306 (2007). 2. Cui, L., et al., J. Histochem. Cytochem. 52: 1447 (2004). 3. Fenderson BA, et al. Methods Mol Biol; 325:207-24 (2006). 4. Harris, J.F., et al., J. Immunol. 132: 2502 (1984).5. Solter, D. and Knowles, B.B., Proc. Natl. Acad. Sci. USA. 75: 5565 (1978).
Additional info: Recognizes mouse SSEA-1. Species Crossreactivity: human.

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