Home  >  Products  >  SRD5A2 (Steroid 5-alpha-reductase 2, 3 oxo 5 alpha Steroid 4 Dehydrogenase 2, 3-oxo-5-alpha-steroid 4-dehydrogenase 2, 5 alpha-SR2, 5-alpha-SR2, e II 5 alpha Reductase, SR Type 2) (Control Peptide)
SRD5A2 (Steroid 5-alpha-reductase 2, 3 oxo 5 alpha Steroid 4 Dehydrogenase 2, 3-oxo-5-alpha-steroid 4-dehydrogenase 2, 5 alpha-SR2, 5-alpha-SR2, e II 5 alpha Reductase, SR Type 2) (Control Peptide)

SRD5A2 (Steroid 5-alpha-reductase 2, 3 oxo 5 alpha Steroid 4 Dehydrogenase 2, 3-oxo-5-alpha-steroid 4-dehydrogenase 2, 5 alpha-SR2, 5-alpha-SR2, e II 5 alpha Reductase, SR Type 2) (Control Peptide)

Cat no: S6505-08R-P

Supplier: United States Biological
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C-KMFEDYPKSRK, from the C-Terminus of human SRD5A2. Control peptide corresponding to polyclonal antibody, Catalog#S6505-08R, goat x human. This gene encodes a microsomal protein expressed at high levels in androgen-sensitive tissues such as the prostate. The encoded protein is active at acidic pH and is sensitive to the 4-azasteroid inhibitor finasteride. Deficiencies in this gene can result in male pseudohermaphroditism, specifically pseudovaginal perineoscrotal hypospadias (PPSH). Applications: Suitable for use in ELISA and Antibody Blocking. Other applications not tested. Recommended Dilution: Antibody Blocking (see corresponding antibody S6505-08R): To start, the best ratio would be 1:1 (which means molar excess of peptides relative to antibodies when identical volumes are mixed). Mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. It is best is to have two identical blots, to be incubated with equal amount of antibodies, but one with the antibodies pre-adsorbed to the peptide for 20min. Then incubate and develop the two blots in parallel. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. P
Catalogue number: S6505-08R-P
Applications: ELISA
Size: 100ug
Form: Supplied as a lyophilized powder in PBS. Reconstitution: Reconstitute with 200ul sterile ddH2O.
Purity: Highly Purified
References: Torkko KC, van Bokhoven A, Mai P, Beuten J, Balic I, Byers TE, Hokanson JE, Norris JM, BarOn AE, Lucia MS, Thompson IM, Leach RJ VDR and SRD5A2 polymorphisms combine to increase risk for prostate cancer in both non-Hispanic White and Hispanic White men. Clin. Cancer Res. 2008 May 14 (10): 3223-9.

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