Src was the first proto-oncogenic non-receptor tyrosine kinase characterized in human. The Src family is composed of nine members in vertebrates, including c-Src, Yes, Fgr, Yrk, Fyn, Lyn, Hck, Lck, and Blk. Src-family kinases transduce signals that are involved in the control of a variety of cellular processes, including proliferation, differentiation, motility, and adhesion. Src-family kinases contain an amino terminal cell membrane anchor followed by an SH3 domain and an SH2 domain. The activity of Src is regulated by tyrosine phosphorylation at multiple sites. Tyrosine 418 is autophosphorylated following Src activation. Tyrosine 215 in the SH-2 domain of Src is phosphorylated following growth factor receptor activation. Both Tyr215 and Tyr418 phosphorylation increases tyrosine kinase activity, while phosphorylation of Tyr529 downregulates Src kinase activity. Thus, tyrosine phosphorylation of Src is critical for regulating its kinase activity.
Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilution:
Western Blot: 1:500-1:1000
Immunohistochemistry (paraffin): 1:50-1:100
Optimal dilutions to be determined by the researcher.
Positive Control:
293 cells
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
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