Suitable for detection and quantitation of Src kinase activity in various cell extracts
Capacity:
~200 assays
Components:
Src Substrate Peptide
Src Reaction Buffer
Src Mn/ATP Cocktail
p81 squares
Other components required but not included as part of kit are:
Enzyme Preparation containing Src Kinase:
10-200ug of immunoprecipitated protein diluted into SrcRB or 2-20U of purified Src Kinase.
vortex mixer
plexiglass shielding
incubating water bath
timer
variable volume (5-200ul)
pipet + tips
phosphoric acid
acetone
scintillation vials
scintillation fluid
scintillation counter
Storage and Stability:
Stable for 1 year at-20 degrees C from date of shipment.
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