Fodrin is a universally expressed membrane-associated cytoskeletal protein consisting of a- and b-subunits. The protein is important for maintaining normal membrane structure and supporting cell surface protein function. a-Fodrin is one of the important targets cleaved by caspases during apoptosis. The full length 240kD protein can be cleaved at several sites within its sequence by activated caspases generating N-terminal 150kD, C-terminal 120kD, and 35kD major products. Cleavage of a-Fodrin leads to membrane malfunction and cell shrinkage
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Western Blot: 0.5-4ug/ml
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.