SPIR1 belongs to the family of Wiskott-Aldrich homology region 2 (WH2) proteins involved in actin organization. The C-terminal region of SPIR1 encodes a zinc finger structure highly homologous to FYVE motifs. The protein also possesses a region highly homologous with other Spir family proteins located adjacent (N-terminal) to the modified FYVE domain and is designated as Spir-box. The protein colocalized specifically with Rab11 GTPase, which is localized at the trans-Golgi network (TGN), post-Golgi vesicles, and the recycling endosome. SPIR1 actin organizer is targeted to intracellular membrane structures by its modified FYVE zinc finger and is involved in vesicle transport processes, providing a novel link between actin organization and intracellular transport. The human SPIR1 gene is localized in the chromosomal region 18p11.21.
Suitable for use in ELISA. Other applications not tested.
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.