Mouse SIGN-R1 is a C-type lectin that has been identified as a murine homologue of the human DC-SIGN related molecule, L-SIGN. Mouse SIGN-R1 is specifically expressed on marginal zone macrophages in the spleen, medullary and subcapsular sinus macrophages in the lymph nodes, and at lower levels on liver sinusoidal endothelial cells. SIGN-R1 has not been detected on murine dendritic cell populations.
Like many C-type lectins, SIGN-R1 functions as a pathogen recognition receptor in vivo and plays an important role in defence against pathogens.
Suitable for use in Flow Cytometry and Immunohistochemistry (frozen). Other applications not tested.
Flow Cytometry: 10ul labels 10e6 cells in 100ul
Immunohistochemistry (frozen): 1:25-1:50
Optimal dilutions to be determined by the researcher.
P3-X63-Ag8.563 myeloma cells with spleen cells from immunized rats.
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.