Home  >  Products  >  solute carrier family 9a3 (Slc9a3, (sodium/hydrogen exchanger), member 3, Na(+)/H(+) exchanger 3, Nhe3, NHE-3, Sodium/hydrogen exchanger 3)
solute carrier family 9a3 (Slc9a3, (sodium/hydrogen exchanger), member 3, Na(+)/H(+) exchanger 3, Nhe3, NHE-3, Sodium/hydrogen exchanger 3)

solute carrier family 9a3 (Slc9a3, (sodium/hydrogen exchanger), member 3, Na(+)/H(+) exchanger 3, Nhe3, NHE-3, Sodium/hydrogen exchanger 3)

Cat no: S5329-10G

Supplier: United States Biological
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Sodium-hydrogen exchanger 3 (NHE3; Slc9a3) is an epithelial transport protein that carries out 1:1 exchange of Na+ and H+ across the plasma membrane. It is apically located in the prroximal tubule of the kidney, the thick ascending limb of the kidney, and in small intestine (1). NHE3 is phosphorylated and regulated by multiple kinases including PKA, SGK1 and CK2. It can be phosphorylated by calyculin A, and dephosphorylated by PP1 catalytic subunit in vitro (2). Applications: Suitable for use in Western Blot and Immunocytochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: S5329-10G
Reactivities: Mouse, Rat
Hosts: Rabbit
Applications: Immunocytochemistry, Western Blot
Size: 100ul
Form: Supplied as a liquid, 50% glycerol.
P type: Pab
Isotype: IgG
Purity: Serum
References: 1. Kim G.H., Ecelbarger C.A., Knepper M.A. and Packer R.K. (1999) J Am Soc Nephrol. 10: 935-942. 2. Dynia D.W., Steinmetz A.G. and Kocinsky H.S. (2010) Am J Physoil Renal Physiol. 298(3): F745-F753.
Additional info: Recognizes rat Slc9a3. Species Crossreactivity: Mouse

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