DPC4 (also named SMAD4; small mothers against decapentaplegic deleted in pancreatic carcinoma). The family of SMAD molecules comprises essential mediators of transforming growth factor beta (TGF-b) signaling. To date, seven members of this family have been identified, each plays a specific and separate role in mediating TGF-b superfamily gene transcription. At least two different members, Smad2 and Smad4/DPC4, have been implicated in human cancer and appear to have tumor-suppressor functions whose inactivation may play an important role in the histogenesis of pancreatic and colorectal malignancy and possibly other human cancers as well.
Suitable for use in Immunoprecipitation, Western Blot and Immunofluorescence. Other applications not tested.
Western Blot: 1-2ug/ml for 2h at RT
Immunoprecipitation: 2ug/mg protein lysate
Optimal dilutions to be determined by the researcher.
LS174T, HT29, raji, jurkat cells
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.