SIRT4, otherwise known as sirtuin 4, is a mitochondrial NAD-dependent ADP-ribosyl transferase, and member of the sirtuin family, which catalyzes the transfer of ADP-ribosyl groups onto target proteins. Studies have shown that SIRT4 acts as a repressor of GDH (glutamate dehydrogenase) activity in beta cell mitochondria, thereby suppressing amino-acid dependent insulin production.
Suitable for use in Western Blot. Other applications not tested.
Western Blot: 1-3ug/ml
Optimal dilutions to be determined by the researcher.
Human Kidney lysate
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.