Siglecs are I-type (Ig-type) lectins belonging to the Ig superfamily. They are characterized by an N-terminal, Ig-like V-type domain that mediates sialic acid binding, followed by varying numbers of Ig-like C2-type domains (2-17), a single transmembrane region, and a cytoplasmic tail. The siglecs can be broadly classified into two subgroups: siglecs-1, -2 and -4, and a siglec-3/CD33-related subgroup (siglecs 3 and -5 through -13 in primates) defined by sequence similarity and clustered gene localization. They are widely expressed on hematopoietic cells, often in a cell-type-specific manner. Siglec 4/MAG is a myelin component in Schwann cells and oligodendrocytes. Their ligands, sialic acids, are negatively charged monosaccharides found on cell-surface glycoproteins and glycolipids. Although siglec functions continue to be defined, most have intracellular immunoreceptor tyrosine-based inhibitory motifs (ITIM), implicating them in the suppression of immunoreceptor signaling. They may also participate in cell/cell interactions or act as receptors for the entry of viral or bacterial pathogens.
Suitable for use in Flow Cytometry. Other applications not tested.
Flow Cytometry: 10ul labels 1x10e5 cells or 50ul of packed whole blood
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C before opening. DO NOT FREEZE! Stable for 12 months at 4 degrees C. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity. PE conjugates are sensitive to light.