The siglecs are a family of membrane bound lectins (of the immunoglobulin superfamily) that bind sialic acid and mediate cell-cell interactions. Family members include sialoadhesin, CD22 and CD33. Sialoadhesin is a macrophage-specific cell adhesion molecule that is expressed on activated macrophages in chronic inflammation and in tumors.
Applications:
Suitable for use in Western Blot, RIA, Flow Cytometry, ELISA and Immunohistochemistry. Other applications not tested.
Recommended Dilutions:
Immunohistochemistry (frozen): 1:10-1:2000
Immunohistochemistry (paraffin): 1:10-1:2000
Immunohistochemistry: 1:10-1:2000
Flow Cytometry: 1:10-1:1000
ELISA: 1:100-1:2000
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.