The siglecs are a family of membrane bound lectins (of the immunoglobulin superfamily) that bind sialic acid and mediate cell-cell interactions. Family members include sialoadhesin, CD22 and CD33. Sialoadhesin is a macrophage-specific cell adhesion molecule that is expressed on activated macrophages in chronic inflammation and in tumors.
Suitable for use in Western Blot, RIA, Flow Cytometry, ELISA and Immunohistochemistry. Other applications not tested.
Immunohistochemistry (frozen): 1:10-1:2000
Immunohistochemistry (paraffin): 1:10-1:2000
Flow Cytometry: 1:10-1:1000
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.