CD169, otherwise known as Sialoadhesin (Sn), is a type I transmembrane glycoprotein and prototypic member of the Siglec (sialic acid binding Ig-like lectin) family, designated Siglec-1.CD169 is a macrophage-restricted interaction molecule which binds to sialylated ligands on haematopoietic cells, including neutrophils, NK cells, monocytes, a subset of CD8+ T cells and B cells, through recognition of sialic acid in the alpha-2,3- glycosidic linkage. CD169 is highly expressed by dendritic cells and on stromal macrophages in the spleen, bone marrow and lymph nodes and to a lesser extent in liver, lungs and gut, and studies have shown high expression of CD169 on macrophages in the inflammatory conditions rheumatoid arthritis and atherosclerosis. Completely inhibits erythrocyte rosetting around Sn+ transductants.
Suitable for use in Flow Cytometry. Other applications not tested.
Flow Cytometry: Neat-1:10 Use 10ul to label 1x10e6 cells in 100ul.
Optimal dilutions to be determined by the researcher.
Spleen cells from immunized Balb/c mice were fused with cells of the X63-Ag8.653 myeloma cell line.
Recommended Negative Control:
I1904-79D:IgG1 Murine Negative Control (PE)
Storage and Stability:
Lyophilized powder may be stored at 4 degrees C. Reconstitute by adding sterile ddH2O. Aliquot and store at 4 degrees C. DO NOT FREEZE! Reconstituted product is stable for 12 months as an undiluted liquid. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity. PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.