High molecular weight serine protease inhibitors (serpins) are large superfamily of proteins, which bind to and inactivate serine proteinases. In 1995 Sprecher et al. cloned 2 novel serpins, termed PI8 and PI9, from human placenta cDNA library. Sequencing showed that PI8 encodes 376aa. PI8 are localized to the cytoplasm of transfected cells and was able to form an SDS- complex. PI8 is implicated to inhibit furin enzymatic activity and plays a role in plateletregulated pathophysiological responses.
Suitable for use in Western Blot. Other applications not tested.
Western Blot: 0.5-4ug/ml
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.