S100 calcium binding protein A10 (S100A10/P11), the protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells. They are involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. The S100 gene group include at least 13 members, which are located as a cluster on chromosome 1q21. This protein may function in exocytosis and endocytosis.
Applications:
Suitable for use in Immunofluorescence, Flow Cytometry, ELISA and Western Blot. Other applications not tested.
Recommended Dilution:
Immunofluorescence: 1:100-1:500
Flow Cytometry: 1:100-1:500
ELISA: 1:10,000
Western Blot: 1:100-1:500
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.