RSK2 is a member of the family of 90-kD ribosomal S6 kinases (1). These proteins are a class of serine/threonine kinases that are activated in response to various extracellular signals, including growth factors, polypeptide hormones and neurotransmitters (2). RSK2 plays a significant role in mediating extracellular signal-related kinase signal transduction. Mutations in RSK2 have been found to be associated with Coffin-Lowry syndrome (3). The N-terminal kinase of RSK2 is involved in substrate phosphorylation. The isolated N-terminal kinase of RSK2 (amino acids 1-360) is phosphorylated at Serine 227 by PDK1. Data indicate that full activation of RSK2 by growth factors requires the cooperation of extracellular signal-regulated kinase and PDK1 through phosphorylation of Serine 227, Serine 369, and Serine 386 (4).
Suitable for use in Western Blotting, Immunoprecipitation. Other applications not tested.
Western Blot: 1:1000-1:2000
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Manufactured incorporating RabMAb(R) technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.