RSK2 is a member of the family of 90-kD ribosomal S6 kinases (1). These proteins are a class of serine/threonine kinases that are activated in response to various extracellular signals, including growth factors, polypeptide hormones and neurotransmitters (2). RSK2 phosphorylates a wide range of substrates, including ribosomal protein S6, and plays a significant role in mediating extracellular signal-related kinase (ERK) signal transduction. Mutations in RSK2 have been found to be associated with Coffin-Lowry syndrome (3).
Suitable for use in Flow Cytometry, Western Blotting, Immunoprecipitation, Immunohistochemistry. Other applications not tested.
Flow Cytometry: 1:50
Western Blotting: 1:5,000 (90kD)
Immunoprecipitation: 1:100 1ug/mg of total cell protein (~10e7 cells)
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Manufactured incorporating RabMAb(R) technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.