Home  >  Products  >  Retinal Pigment Epithelium Specific Protein (Retinal Pigment Epithelium-specific 65kD Protein, RPE65, All-trans-retinyl-palmitate Hydrolase, LCA2, mRPE65, rd12, Retinol Isomerase, Retinoid Isomerohydrolase, RP20, sRPE65) (HRP)

Retinal Pigment Epithelium Specific Protein (Retinal Pigment Epithelium-specific 65kD Protein, RPE65, All-trans-retinyl-palmitate Hydrolase, LCA2, mRPE65, rd12, Retinol Isomerase, Retinoid Isomerohydrolase, RP20, sRPE65) (HRP)

Cat no: R1659-70D


Supplier: United States Biological
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Highly conserved among vertebrate species, RPE65 is a major protein of the retinal pigment epithelium (RPE). RPE65 is essential for the regeneration of rhodopsin in the visual cycle. Mutations in RPE65 are responsible for certain forms of autosomal recessive severe retinal dystrophy, including Leber Congenital Amaurosis (LCA). Loss of function results in a block in retinoid processing required for the synthesis of 11-cis retinal and the accumulation of retinyl esters in the RPE. Applications: Suitable for use in Western Blot, Immunohistochemistry and Immunocyctochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 1:5000-1:10,000, recognizes a band at 65kD, representing bovine RPE65 protein. Immunohistochemistry: 1:250 Optimal dilutions to be determined by researcher. Storage and Stability: Store at 2-8 degrees C. Do Not Freeze! Aliquots are stable for at least 3 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
Catalogue number: R1659-70D
Reactivities: Bovine
Hosts: Mouse
Applications: Immunocytochemistry, Immunohistochemistry, Western Blot
Size: 100ul
Form: Supplied as a liquid in PBS, pH 7.4. Labeled with HRP.
P type: Mab
Isotype: IgG1,k
Purity: Purified by Protein A affinity chromatography.
Alternative names: Retinal Pigment Epithelium-specific Protein 65kD, EC=3.1.1.64, EC=5.2.1.7, Leber Congenital Amaurosis, Retinitis Pigmentosa 20
References: 1. Jacobson, A., C. Yan, et al. (2007) 293:H1344-1350. 2. Jacobson, S. G., T. S. Aleman, et al. (2007) 104:15123-15128. 3. Jiang, G., et al. (2008) Invest. Ophthalmol. Vis. Sci. Jan 49:282-289. 4. Lall, M. M., et al. (2008) Invest. Ophthalmol. Vis. Sci. Jan 49:310-319. 5. Chen M, et al. (2008) Invest Ophthalmol Vis Sci 49(8):3699-3706. 6. Harris JR, et al. (2008) Stem Cells 2008-0836. 7. Nicoletti, A., et al. (1995) Hum. Mol. Genet. 4:641-649. 8. Thompson, D.A., et al. (2000) Invest. Ophthalmol. Vis. Sci. 41:4293-42999. 9. Redmond, T.M. et al. (1998) Nat. Genet. 20:344-351. 10. Hamel, C.P., et al. (1993) JBC. 268(21):15751-15757.
Additional info: Recognizes RPE65. Tested in bovine, porcine, human and murine.

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