Home  >  Products  >  Retinal Pigment Epithelium Specific Protein (Retinal Pigment Epithelium-specific 65kD Protein, RPE65, All-trans-retinyl-palmitate Hydrolase, LCA2, mRPE65, rd12, Retinol Isomerase, Retinoid Isomerohydrolase, RP20, sRPE65) (Biotin)
Retinal Pigment Epithelium Specific Protein (Retinal Pigment Epithelium-specific 65kD Protein, RPE65, All-trans-retinyl-palmitate Hydrolase, LCA2, mRPE65, rd12, Retinol Isomerase, Retinoid Isomerohydrolase, RP20, sRPE65) (Biotin)

Retinal Pigment Epithelium Specific Protein (Retinal Pigment Epithelium-specific 65kD Protein, RPE65, All-trans-retinyl-palmitate Hydrolase, LCA2, mRPE65, rd12, Retinol Isomerase, Retinoid Isomerohydrolase, RP20, sRPE65) (Biotin)

Cat no: R1659-70B

Supplier: United States Biological
Star_fadedStar_fadedStar_fadedStar_fadedStar_faded
0 reviews | Write a Review Pencil
Read More on Supplier Website
Highly conserved among vertebrate species, RPE65 is a major protein of the retinal pigment epithelium (RPE). RPE65 is essential for the regeneration of rhodopsin in the visual cycle. Mutations in RPE65 are responsible for certain forms of autosomal recessive severe retinal dystrophy, including Leber Congenital Amaurosis (LCA). Loss of function results in a block in retinoid processing required for the synthesis of 11-cis retinal and the accumulation of retinyl esters in the RPE. Applications: Suitable for use in Western Blot, Immunohistochemistry and Immunocyctochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 1:5000-1:10,000, recognizes a band at 65kD, representing bovine RPE65 protein. Immunohistochemistry: 1:250 Optimal dilutions to be determined by researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degrees C. Aliquots are stable for at least 3 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: R1659-70B
Reactivities: Bovine
Hosts: Mouse
Applications: Immunocytochemistry, Immunohistochemistry, Western Blot
Conjugates: Biotin
Size: 100ul
Form: Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide. Labeled with Biotin.
P type: Mab
Isotype: IgG1,k
Purity: Purified by Protein A affinity chromatography.
Alternative names: Retinal Pigment Epithelium-specific Protein 65kD, EC=3.1.1.64, EC=5.2.1.7, Leber Congenital Amaurosis, Retinitis Pigmentosa 20
References: 1. Jacobson, A., C. Yan, et al. (2007) 293:H1344-1350. 2. Jacobson, S. G., T. S. Aleman, et al. (2007) 104:15123-15128. 3. Jiang, G., et al. (2008) Invest. Ophthalmol. Vis. Sci. Jan 49:282-289. 4. Lall, M. M., et al. (2008) Invest. Ophthalmol. Vis. Sci. Jan 49:310-319. 5. Chen M, et al. (2008) Invest Ophthalmol Vis Sci 49(8):3699-3706. 6. Harris JR, et al. (2008) Stem Cells 2008-0836. 7. Nicoletti, A., et al. (1995) Hum. Mol. Genet. 4:641-649. 8. Thompson, D.A., et al. (2000) Invest. Ophthalmol. Vis. Sci. 41:4293-42999. 9. Redmond, T.M. et al. (1998) Nat. Genet. 20:344-351. 10. Hamel, C.P., et al. (1993) JBC. 268(21):15751-15757.
Additional info: Recognizes RPE65. Tested in bovine, porcine, human and murine.

Get Quote

  • Best Price Guaranteed
  • Quick Response Time
  • Exclusive Promotions
Enquiry_down_arrow
United States Biological
Get a Quote Direct from
United States Biological

By submitting this form you agree to your details being passed to United States Biological for the purpose of generating the best quote*
See Full Product Listings >
Button_on Button_off_biosave Button_off_biosave Button_off_biosave Button_off_biosave Button_off_biosave Button_off_biosave