This kit is a biotin-labeled-antibody-based sandwich enzyme immunoassay for the quantitative measurement of mouse resistin in serum and tissue culture medium. It is intended for in vitro and research use only.
In the Mouse Resistin ELISA kit, calibrators or samples are incubated with a goat polyclonal anti-mouse resistin antibody coated in microtiter wells. The wells are washed and biotin-labeled polyclonal anti-mouse resistin antibody is added and incubated with captured resistin. After a thorough wash, streptavidin-horseradish peroxidase conjugate is added. After the subsequent incubation and washing steps, the conjugate bound is allowed to react with the substrate H2O2-tetramethylbenzidine. The reaction is stopped by the addition of acidic solution and the absorbance of the resulting yellow product is measured at 450nm. The absorbance is proportional to the concentration of resistin. A standard curve is constructed by plotting the absorbance values versus the resistin concentrations of the calibrators, and the concentrations of unknown samples are determined using this standard curve.
R1585-01K1: Microtiter Plate, 1x96 wells
R1585-01K2: Pab (Biotin) 1x13ml
R1585-01K3: Streptavidin (HRP), 1x13ml
R1585-01K4: Standard, 100ng/ml, 1x50ul
R1585-01K5: Standard, 50ng/ml, 1x50ul
R1585-01K6: Standard, 20ng/ml, 1x50ul
R1585-01K7: Standard, 10ng/ml, 1x50ul
R1585-01K8: Standard, 5ng/ml, 1x50ul
R1585-01K9: Standard, 2ng/ml, 1x50ul
R1585-01K10: Standard, 1ng/ml, 1x50ul
R1585-01K11: Quality Control, (18-27ng/ml), 1x1vial
R1585-01K12: Dilution Buffer (5X), 1x22ml
R1585-01K13: Wash Buffer (10X), 1x100ml
R1585-01K14: Substrate Solution (TMB), 1x13ml
R1585-01K15: Stop Solution, H2SO4, 1x13ml
Storage and Stability:
Store standards *R1585-01K4-R1585-01K10 at 4 degrees C; when diluted, aliquot and store at -20 degrees C. Store R1585-01K11 powder at 4 degrees C, liquid at -20 degrees C. Store other components at 4 degrees C. Stable for at least 3 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.