Home  >  Products  >  RAF1, Phosphorylated (Ser259) (Raf-1, RAF Proto-oncogene Serine/Threonine-protein Kinase, Proto-oncogene c-RAF, cRaf, RAF)

RAF1, Phosphorylated (Ser259) (Raf-1, RAF Proto-oncogene Serine/Threonine-protein Kinase, Proto-oncogene c-RAF, cRaf, RAF)

Cat no: R0495-06B


Supplier: United States Biological
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c-Raf is a mitogen-activated protein kinase. Binding of Raf to 14-3-3 is required for c-Raf kinase activity, and also blocks the ability of phosphatase to inhibit c-Raf. 14-3-3 binds c-Raf at two phospho-serine residues: Ser259 and Ser621. Mutation of Ser259 to alanine constitutively activates Raf kinase activity, but mutation of Ser621 to alanine results in an inactive kinase. Applications: Suitable for use in Immunohistochemistry and Western Blot. Other applications not tested. Recommended Dilution: Immunohistochemistry: 20-40ug/ml Western Blot: 0.5-4ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: R0495-06B
Reactivities: Human, Mouse, Rat
Hosts: Rabbit
Applications: Immunohistochemistry, Western Blot
Size: 100ug
Form: Supplied as a liquid in PBS, pH 7.2, 1% BSA, 0.02% thimerosal, 50% glycerol.
P type: Pab
Isotype: IgG
Purity: Purified by affinity chromatography.
Additional info: Recognizes human Raf at ~74kD when phosphorylated at Ser259. Species Crossreactivity: mouse and rat.

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