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Rac1 Activation Assay Kit, BioAssay(TM)

Rac1 Activation Assay Kit, BioAssay(TM)

Cat no: R0400-55

Supplier: United States Biological
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Kit Components R0400-55A: PAK-1 PBD, agarose. 1x300ug of PAK-1 PBD bound to 150ul of glutathione agarose beads, provided as a 50% slurry in 20mM PBS, pH 7.4, containing 50% glycerol for a final volume of 300ul. R0400-55B: Rac1 Mab: 1x250ug in 250ul of 0.1M Tris-glycine, pH 7.4, 0.15M NaCl, containing 0.05% sodium azide. Protein G purified mouse IgG2b. R0400-55C: Mg2+ Lysis/Wash Buffer, 5X, 2x18ml Supplied as a liquid in125mM HEPES, pH 7.5, 750mM NaCl, 5% Igepal CA-630, 50mM MgCl2, 5mM EDTA and 10% glycerol. R0400-55D: GTPgS (100X) 1x50ul of 10mM GTPgS, 100X stock, in sterile, distilled water. Non-hydrolyzable analog of GTP. Sufficient to label 5ml of cell lysates. R0400-55E: GDP (100X) 1x50ul of 100mM GDP, 100X stock, in sterile, distilled water. GDP (Guanosine 5
Catalogue number: R0400-55
Size: 1Kit
References: 1. Wei, Q. and Adelstein, R. S., Mol. Biol. Cell 13: 683-697, 2002. 2. Cook, J. A., et al, J. Biol. Chem. 278: 35812-35818, 2003. 3. Taylor, S.J. and D. Shalloway, Current Biology 6: 1621-1627, 1996.

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