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Rab5 (Ras-related Protein Rab-5A, RAB5A)

Cat no: R0008-01F


Supplier: United States Biological
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Rab5 is a 24kD GTP-binding protein that is localized to the cytoplasmic side of the plasma membrane, clathrin-coated vesicles, and early endosomes. Rab5 is believed to regulate vesicle fusion through a cycle of GDP/GTP exchange and GTP hydrolysis. The different guanine nucleotide binding states of rab5 is postulated to affect its ability to associate or dissociate with membranes during endocytotic membrane traffic. Its GTP-bound form, which represents the active form of Rab5, associates with membrane and regulates vesicle docking and fusion. Applications: Suitable for use in Western Blot, Immunohistochemistry and Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 0.5-4ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: R0008-01F
Reactivities: Human, Mouse, Rat, Bovine, Non-Human Primate
Hosts: Rabbit
Applications: Immunohistochemistry, Immunoprecipitation, Western Blot
Size: 100ug
Form: Supplied as a liquid in PBS, 0.5% BSA, 0.01% thimerosal, 30% glycerol.
P type: Pab
Isotype: IgG
Purity: Purified by affinity chromatography.
Additional info: Recognizes human Rab5 at ~26kD. Species Crossreactivity: mouse, rat, monkey and bovine.

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