5'-C T G C A^G-3'
3'-G^A C G T C-5'
Concentration: 10 units/ul (see P9180-05 for 50u/ul)
Source:
Providencia stuarti
Supplied With:
R1625: Restriction Enzyme Buffer A, 10X
R1625-03: Restriction Enzyme Buffer D, 10X
Concentration:
10u/ul
Incubation Temperature:
37 degrees C
Unit Definition:
One unit is defined as the amount of PstI required to digest 1ug of lambda DNA in 1 hour at 37 degrees C in 50ul of assay buffer.
Diluent Buffer:
10mM Tris-HCl pH 7.4,100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA, 50% glycerol. For longer periods, the Storage Buffer should be used.
Storage Buffer:
Supplied as a liquid in 10mM Tris-HCl pH 7.4, 200mM sodium chloride, 1mM DTT, 0.1mM EDTA, 0.15% Triton X-100, 0.2mg/ml BSA, 50% glycerol.