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PstI 10u/ul

Cat no: P9180


Supplier: United States Biological
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5'-C T G C A^G-3' 3'-G^A C G T C-5' Concentration: 10 units/ul (see P9180-05 for 50u/ul) Source: Providencia stuarti Supplied With: R1625: Restriction Enzyme Buffer A, 10X R1625-03: Restriction Enzyme Buffer D, 10X Concentration: 10u/ul Incubation Temperature: 37 degrees C Unit Definition: One unit is defined as the amount of PstI required to digest 1ug of lambda DNA in 1 hour at 37 degrees C in 50ul of assay buffer. Diluent Buffer: 10mM Tris-HCl pH 7.4,100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA, 50% glycerol. For longer periods, the Storage Buffer should be used. Storage Buffer: Supplied as a liquid in 10mM Tris-HCl pH 7.4, 200mM sodium chloride, 1mM DTT, 0.1mM EDTA, 0.15% Triton X-100, 0.2mg/ml BSA, 50% glycerol.
Catalogue number: P9180
Size: 10,000U

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