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Protein MW Marker, Prestained, 6-175kD

Cat no: P9106-75

Supplier: United States Biological
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Prestained Protein Marker, Broad Range is a mixture of purified proteins covalently coupled to a blue dye that resolves to 8 bands between 6-175kD when electrophoresed (1). The protein concentrations are carefully balanced for even intensity. The covalent coupling of the dye to the proteins affects their electrophoretic behavior in SDS-PAGE gels relative to unstained proteins. Recommended Usage: 1. Transfer the desired amount of the Prestained Protein Marker to a separate tube. For visualization during electrophoresis: Mini-gels: use15ul. Full size gels: use 30ul. 2. Heat the Marker to 95-100 degrees C for 3-5 minutes. 3. Microcentrifuge briefly. Load directly on to the gel. To ensure uniform mobility, load an equal volume of 1X rSDS Reducing Loading Buffer into any unused wesll. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: P9106-75
Size: 70 Blots
Form: Supplied as a liquid in 70mM Tris-HCl, pH 6.8 at 25 degrees C, 33 mM sodium chloride, 1mM Na2EDTA, 2% SDS, 40mM dithiothreitol (DTT), 0.01% phenol red, 10% glycerol.
References: 1. Sambrook, J., Fritsch, E.F., Maniatis, T. Molecular Cloning: A Laboratory Manual, second edition, Cold Spring Harbor Laboratory (1989). 2. Laemmli, U.K., Nature 227: 680-685 (1970).
Additional info: Apparent Protein Source MW (kD) MBP-b-galactosidase* E. coli 175.0 MBP-paramyosin* E. coli 83.0 Glutamic dehydrogenase bovine liver 62.0 Aldolase rabbit muscle 47.5 Triosephosphate isomerase rabbit muscle 32.5 b-Lactoglobulin A bovine milk 25.0 Lysozyme chicken egg white 16.5 Aprotinin bovine lung 6.5 *MBP = maltose-binding protein. MBP-b-galactosidase = fusion of MBP and b-galactosidase. MBP-paramyosin = fusion of MBP and paramyosin.

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