Protein MW Marker detection packs are designed to detect molecular weight ladders on Western Blots when using the horseradish peroxidase (HRP) based Western detection system. The pack contains biotinylated protein ladders and HRP-linked anti-biotin antibody. The molecular weight ladders are a mixture of purified proteins covalently coupled to biotin that resolve to 10 bands that have a size range of 9-200kD. The anti-biotin antibody is used to detect biotinylated protein ladders on Western blots. The packs have been optimized for chemiluminescent Western detection procedures.
The protein ladder consists of 10 proteins ranging in apparent molecular weight from 9-200kD. The 9kD protein is derived from aprotinin purified from bovine lung. The proteins from 20-50kD are paramyosin fragments. The higher molecular weight proteins are fusions of maltose binding protein (MBP) with paramyosin or paramyosin/lacZ fragments.
P9106-25A, Prestained Protein Marker, Broad Range: Supplied as a liquid in 65mM Tris-HCl, pH 7.0 , 35mM sodium chloride, 1mM Na2EDTA, 2% SDS, 1mM sodium azide, 40mM dithiothreitol (DTT), 0.01% phenol red, 10% glycerol.
P9106-25B, Biotin (HRP): Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
Molecular Weight (kD):
200,140,100, 80, 60, 50, 40, 30, 20, 9