Turbo Broth(TM) and Turbo Prime Broth(TM) are proprietary media formulations used for the cultivation of recombinant strains of E. coli. Turbo Prime Broth(TM) is made without animal-derived raw materials and is APF Certified. These media have been developed to improve the yield of recombinant proteins. The carbon source is glycerol which is not metabolized into acetic acid. It has a rich nutrient base which provides amino acids, vitamins, inorganic and trace minerals at levels higher than those of LB Broth. The medium is buffered at pH 7.2