Protein Expression Media Kit, pERK(TM) (Powder) pEX(TM)

Kit Components Individually packaged P9102-41B1: LB Broth (Miller) 1x1L P9102-41B2: Glucose M9Y 1x1L P9102-41B3: Turbo BrothTM 1x1L P9102-41B4: Glucose Nutrient Mix 1x100ml P9102-41B5: Superior BrothTM 1x1L P9102-41B6: LB*Booster, 20X 1x20ml P9102-41B7: Power BrothTM 1x1L P9102-41B8: AugmediumTM, 50X 1x25ml P9102-41B9: Hyper BrothTM 1x1L Storage: Store powdered media at RT, except store P9102-41B8 powder at -20 degrees C. Prepared media should be kept at 4 degrees C and used within a short period of time. The Protein Expression Media Kit is intended to help maximize the amount of soluble protein produced during expression of recombinant proteins in E. coli. This comprehensive kit includes samples of Expression Media and two reference media, Glucose M9Y and LB (Miller) Broth. The Expression Media are specially designed to increase the production of recombinant proteins. The kit also includes new medium additives: LB Booster and Augmedium. LB Booster is designed to increase the bio-mass production of any expression medium up to 5-fold. Augmedium is a medium pre-conditioner that induces the expression of chaperone proteins in advance of expression of the target recombinant protein. This can increase the amount of soluble protein accumulated. Method: Helps to maximize the amount of soluble protein produced during expression of recombinant proteins in E. coli. Preparation: Reagents Dissolve the contents of the LB Booster packet in 20mL deionized water and filter sterilize using a 0.2um filter. The stock solution will be 20x. Store at 4 degrees C. Dissolve the contents of the Augmedium container in 25mL deionized water and fi lter sterilize using a 0.2um filter. Aliquot 5mL portions into sterile 15mL conical tubes and store at -20 degrees C. The stock will be a 50x solution. For use, snap thaw at 37 degrees C and store at 4 degrees C for no more than five (5) days. pEX(TM) is a trademark of United States Biological Protein Expression Rescue Kit(TM) is a trademark of Athena Enzyme Systems(TM)