Hyper Broth(TM) is a proprietary medium formulation for improved expression of recombinant proteins in E. coli. The medium is a complex, rich formulation which supplies amino acids, vitamins and glucose at high levels. It is made without the use of animal-derived raw materials and is APF Certified. This medium yields the highest level of biomass of all AthenaES(TM) media. The medium is buffered at pH 7.3