PSMA (prostate specific membrane antigen) is a 100-120kD type II, integral membrane glycoprotein,1 composed of a 19 amino acid intracellular domain containing the N-terminus, a 24 amino acid transmembrane region, and a 707 amino acid extracellular C-terminal domain. The PSMA gene has been cloned and sequenced, and has been localized to chromosome 11q. Three functionally distinct proteins are encoded, including folylpoly-gamma-glutamate carboxypeptidase in the intestine, N-acetylated alpha-linked acidic dipeptidase 1 in the brain, and prostate-specific membrane antigen in the prostate.
Applications:
Suitable for use in ELISA, Immunofluorescence and Western Blot. Other applications not tested.
Recommended Dilution:
Western Blot: 1:2000
Immunofluorescence: 1:200-1:300
Optimal dilutions to be determined by the researcher.
Hybridoma:
FO myeloma cells with spleen cells from Balb/c mice.
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.