Prostate specific antigen (PSA) belongs to the kallikrein gene family. It is a single-chain glycoprotein with a molecular mass of about 33kD which is produced by prostate epithelial cells. It is secreted in the seminal plasma and is responsible for liquefaction of the seminal coagulum. PSA is present in small quantities in the serum of healthy males, and is often elevated in the presence of prostate cancer and in other prostate disorders. It is one of the most useful tumor markers because of its tissue specificity. It is thus widely used for screening, diagnosis, and monitoring patients with prostate cancer.
Applications:
Suitable for use in Flow Cytometry, Immunohistochemistry. Other applications not tested.
Recommended Dilution:
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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