The peptide Polymyxin B (PMB) is a cyclic amphipathic peptide antibiotic that binds to endotoxins (lipopolysaccharide, LPS). Binding of PMB to LPS results in neutralization of LPS of gram-negative bacteria. For the study of PMB-LPS interactions PMB conjugates proved to be superior to the application of anti-PMB antibodies. PMB conjugates are useful general probes to detect or measure lipid A and LPS of various species using simple methods. These conjugates are also useful for staining of bacteria. The conjugates obviate the need for many specific antisera. Furthermore PMB conjugates are useful tools in investigations of LPS binding protein (LBP) and bactericidal permeability increasing protein (BPI) interactions with LPS. PMB conjugates are useful probes for endotoxin research.
Applications:
Suitable for use in immuno assays and biological assays. Other applications not tested.
Recommended Dilution:
Optimal dilutions to be determined by the researcher.
Storage and Stability:
Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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