Plectin, a 500kD intermediate filament binding protein, is localized to the cytoplasm and acts to both stabilize and give strength to cells via cross-linking (1). Plectin cross-links intermediate filaments to microtubules and microfilaments, and anchors intermediate filaments to the plasma and nuclear membranes (2,3). Plectin binds many proteins, including actin, vimentin, and Lamin B. Phosphorylation by PKA or PKC results in decreased binding to Lamin B, and phosphorylation by PKA enhances the plectin-vimentin interactions (4).
Plasma membrane attachment sites of intermediate filaments and microfilaments (actin) such as desmosomes and hemidesmosomes, Z-line structures and dense plaques of striated and smooth muscle. Strong positive reaction is also found with endothelia, fibroblasts and infiltrating cells (e.g. lymphocytes).
Applications:
Suitable for use in Western Blot, Immunohistochemistry. Other applications not tested.
Recommended
Immunohistochemistry (frozen sections (5-10ug/ml).
Western Blot (2ug/ml)
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50?, aliquot and store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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