PDGF was originally discovered as a major mitogenic factor in serum, but not in plasma. PDGF is stored in platelet alpha granules and released upon platelet activation. Besides megakaryocytes, other cell types, including endothelial cells, monocyte/macrophages, vascular smooth muscle cells, fibroblasts, cytotrophoblasts, and a variety of transformed or neoplastic cells, have been shown to produce PDGF. PDGFs are disulfide-linked dimers. The subunits of the PDGF dimer are homologous polypeptides designated PDGF-A and PDGF-B chains. Natural PDGFs can exist either as homodimers (PDGF-AA, PDGF-BB) or heterodimers (PDGF-AB). Two distinct PDGF receptors, the a-receptor and the b-receptor, have been identified. The two receptors are structurally related, with an extracellular portion containing five immunoglobulin-like domains, a single transmembrane region, and an intracellular portion with a protein-tyrosine kinase domain. The a-receptor binds both the A and B chains with high affinity whereas the b-receptor binds only the B-chain with high affinity. Receptor dimerization is induced upon ligand binding. In addition to being a potent mitogen for cells of mesenchymal origin, PDGF has also been shown to be a potent chemoattractant for mesenchymal cells, mononuclear cells, and neutrophils and has been reported to be important in the modification of cellular matrix constituents.
Suitable for use in Flow Cytometry. Other applications not tested.
Flow Cytometry: 10ul labels 1x10e6 cells
Optimal dilutions to be determined by the researcher.
Recommended Control Antibodies:
I1904-75D: IgG1 (FITC) Pab Rb x Mo
I1904-75N: IgG1 (FITC) Mab Rt x Mo
I1904-79L: IgG1 (FITC) Pab Gt x Mo
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.