Home  >  Products  >  Platelet Derived Growth Factor Receptor, Type B (PDGFRb) (Carboxyfluorescein)

Platelet Derived Growth Factor Receptor, Type B (PDGFRb) (Carboxyfluorescein)

Cat no: P4258-23C

Supplier: United States Biological
0 reviews | Write a Review Pencil
PDGF was originally discovered as a major mitogenic factor in serum, but not in plasma. PDGF is stored in platelet alpha granules and released upon platelet activation. Besides megakaryocytes, other cell types, including endothelial cells, monocyte/macrophages, vascular smooth muscle cells, fibroblasts, cytotrophoblasts, and a variety of transformed or neoplastic cells, have been shown to produce PDGF. PDGFs are disulfide-linked dimers. The subunits of the PDGF dimer are homologous polypeptides designated PDGF-A and PDGF-B chains. Natural PDGFs can exist either as homodimers (PDGF-AA, PDGF-BB) or heterodimers (PDGF-AB). Two distinct PDGF receptors, the a-receptor and the b-receptor, have been identified. The two receptors are structurally related, with an extracellular portion containing five immunoglobulin-like domains, a single transmembrane region, and an intracellular portion with a protein-tyrosine kinase domain. The a-receptor binds both the A and B chains with high affinity whereas the b-receptor binds only the B-chain with high affinity. Receptor dimerization is induced upon ligand binding. In addition to being a potent mitogen for cells of mesenchymal origin, PDGF has also been shown to be a potent chemoattractant for mesenchymal cells, mononuclear cells, and neutrophils and has been reported to be important in the modification of cellular matrix constituents. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 10ul labels 1x10e6 cells Optimal dilutions to be determined by the researcher. Recommended Control Antibodies: I1904-75D: IgG1 (FITC) Pab Rb x Mo I1904-75N: IgG1 (FITC) Mab Rt x Mo I1904-79L: IgG1 (FITC) Pab Gt x Mo Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: P4258-23C
Reactivities: Human, Non-Human Primate
Hosts: Mouse
Applications: Flow Cytometry
Size: 100Tests
Form: Supplied as a liquid in PBS, 0.5% BSA, 0.1% sodium azide. Labeled with Carboxyfluorescein.
P type: Mab
Isotype: IgG1
Purity: Purified by Protein G affinity chromatography.
Additional info: Recognizes the human Platelet Derived Growth Factor Receptor, Type B. Does not recognize the PDGF Receptor Type A. Species Crossreactivity: monkey. Does not crossreact with rat or mouse receptors.

Get Quote

  • Best Price Guaranteed
  • Quick Response Time
  • Exclusive Promotions
United States Biological
Get a Quote Direct from
United States Biological

By submitting this form you agree to your details being passed to United States Biological for the purpose of generating the best quote*

Button_on Button_off_biosave Button_off_biosave Button_off_biosave Button_off_biosave Button_off_biosave Button_off_biosave