PDGF was originally discovered as a major mitogenic factor in serum but not in plasma. PDGF is stored in platelet a granules and released upon platelet activation. Besides megakaryocytes, other cell types, including endothelial cells, monocyte/macrophages, vascular smooth muscle cells, fibroblasts, cytotrophoblasts, and a variety of transformed or neoplastic cells, have been shown to produce PDGF. PDGFs are disulfide-linked dimers. The subunits of the PDGF dimer are homologous polypeptides designated PDGF-A and PDGF-B chains. Natural PDGFs can exist either as homodimers (PDGF-AA, PDGF-BB) or heterodimers (PDGF-AB). Two distinct PDGF receptors, the a-receptor and the b-receptor, have been identified. The two receptors are structurally related, with an extracellular portion containing five immunoglobulin-like domains, a single transmembrane region, and an intracellular portion with a protein-tyrosine kinase domain. The a-receptor binds both the A and B chains with high affinity whereas the b-receptor binds only the B-chain with high affinity. Receptor dimerization is induced upon ligand binding. In addition to being a potent mitogen for cells of mesenchymal origin, PDGF has also been shown to be a potent chemoattractant for mesenchymal cells, mononuclear cells, and neutrophils and has been reported to be important in the modification of cellular matrix constituents.
Applications:
Suitable for use in Flow Cytometry. Other applications not tested.
Recommended Dilution:
Flow cytometry: 10ul lables 1x10e6 cells
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C before opening. DO NOT FREEZE! Stable at 4 degrees C. Stable for 12 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Freezing APC conjugates will result in a substantial loss of activity. APC conjugates are sensitive to light.
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