Home  >  Products  >  Peroxidase, Horseradish (Horseradish Peroxidase, HRP, EC 1.11.1.7, HRPA2, HPRC1, HRPC2, HRPE5, HRPN, Neutral Peroxidase, Peroxidase A2, Peroxidase C1A, PRXC1A, Peroxidase C1B, PRXC1B, Peroxidase C2, PRXC2, Peroxidase C3, PRXC3, Peroxidase E5, Peroxidase N
Peroxidase, Horseradish (Horseradish Peroxidase, HRP, EC 1.11.1.7, HRPA2, HPRC1, HRPC2, HRPE5, HRPN, Neutral Peroxidase, Peroxidase A2, Peroxidase C1A, PRXC1A, Peroxidase C1B, PRXC1B, Peroxidase C2, PRXC2, Peroxidase C3, PRXC3, Peroxidase E5, Peroxidase N

Peroxidase, Horseradish (Horseradish Peroxidase, HRP, EC 1.11.1.7, HRPA2, HPRC1, HRPC2, HRPE5, HRPN, Neutral Peroxidase, Peroxidase A2, Peroxidase C1A, PRXC1A, Peroxidase C1B, PRXC1B, Peroxidase C2, PRXC2, Peroxidase C3, PRXC3, Peroxidase E5, Peroxidase N

Cat no: P3350-08A

Supplier: United States Biological
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Horseradish Peroxidase (HRP) is an enzyme commonly used as an indicator for chemical reactions which produce peroxide. The enzyme is routinely conjugated to antibodies for use in enzyme-base immunoassay systems. HRP functions in the removal of hydrogen peroxide, oxidation of toxic reductants, biosynthesis and degradation of lignin, suberization, auxin catabolism, response to environmental stresses such as wounding, pathogen attack and oxidative stress. These functions might be dependent on each isozyme/isoform in each plant tissue. HRP binds one heme B (iron-protoporphyrin IX) group and two calcium ions per monomeric subunit. Applications: Suitable for use in ELISA, Western Blot, Flow Cytometry, FACS Analysis and Immunocytochemistry. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: P3350-08A
Hosts: Mouse
Applications: ELISA, Flow Cytometry, Immunocytochemistry, Western Blot
Size: 100ug
Form: Supplied as a liquid in PBS, pH 7.4, 15mM sodium azide, before the addition of glycerol to 40%.
P type: Mab
Isotype: IgG1
Purity: Purified by immunoaffinity chromatography.
References: Viklicky V et al. Monoclonal antibodies against horseradish peroxidase. Folia Biol (Praha) 30:377-82 (1984).
Additional info: Recognizes horseradish peroxidase (HRP).

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