Home  >  Products  >  PDI (Protein Disulfide Isomerase, Cellular Thyroid Hormone Binding Protein, Disulphide Isomerase, DSI, ERBA2L, Glutathione Insulin Transhydrogenase, GIT, p55, PDIA1, PDIP, PDIR, PHDB, Procollagen-proline 2-oxoglutarate 4-dioxygenase beta, PO4DB, PO4HB, Pr

PDI (Protein Disulfide Isomerase, Cellular Thyroid Hormone Binding Protein, Disulphide Isomerase, DSI, ERBA2L, Glutathione Insulin Transhydrogenase, GIT, p55, PDIA1, PDIP, PDIR, PHDB, Procollagen-proline 2-oxoglutarate 4-dioxygenase beta, PO4DB, PO4HB, Pr

Cat no: P3122-05D


Supplier: United States Biological
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Protein disulfide isomerase (PDI) is an acidic 55kD thyroid hormone-binding protein that appears to be identical to the glial-p55 protein. It has been suggested to be involved in disulfide-bond formation and isomerization, as well as the reduction of disulfide bonds in proteins. It may also play a role in the thyroid hormone-dependent regulation of actin polymerization and the degradation of the membrane-associated protein type II iodothyronine 5'-deiodinase (5'D-II). Applications: Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Immunohistochemistry (Paraffin): 1:100. Antigen retrieval not essential but may optimize staining. Staining in rat intestine yields a pattern consistent with cytoplasmic staining. Western Blot: 1:1000. Detects a band of ~59kDa representing PDI from rat liver extract or a slightly higher protein at 61kDa representing PDI from human liver extract. Immunoprecipitation: Inhibits the activity of PDI in vitro. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: P3122-05D
Reactivities: Human, Mouse, Rat
Hosts: Mouse
Applications: Immunohistochemistry, Immunoprecipitation, Western Blot
Size: 100ul
Form: Supplied as a liquid, 0.05% sodium azide.
P type: Mab
Isotype: IgG2b
Purity: Ascites
References: 1. Basu S & Srivastava PK Calreticulin, a peptide-binding chaperone of the endoplasmic reticulum, elicits tumor- and peptide-specific immunity. J Exp Med 189:797-802 (1999). 2. Kuznetsov G et al. Several endoplasmic reticulum stress proteins, including ERp72, interact with thyroglobulin during its maturation. J Biol Chem 269:22990-5 (1994). 3. Kaetzel CS et al. Protein disulphide-isomerase from human placenta and rat liver. Purification and immunological characterization with monoclonal antibodies. Biochem J 241:39-47 (1987).
Additional info: Recognizes rat PDI. Species Crossreactivity: rat and mouse tissues, hamster cells.

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