Protein disulfide isomerase (PDI) is an acidic 55kD thyroid hormone-binding protein that appears to be identical to the glial-p55 protein. It has been suggested to be involved in disulfide-bond formation and isomerization, as well as the reduction of disulfide bonds in proteins. It may also play a role in the thyroid hormone-dependent regulation of actin polymerization and the degradation of the membrane-associated protein type II iodothyronine 5'-deiodinase (5'D-II).
Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.
Immunohistochemistry (Paraffin): 1:100. Antigen retrieval not essential but may optimize staining. Staining in rat intestine yields a pattern consistent with cytoplasmic staining.
Western Blot: 1:1000. Detects a band of ~59kDa representing PDI from rat liver extract or a slightly higher protein at 61kDa representing PDI from human liver extract.
Immunoprecipitation: Inhibits the activity of PDI in vitro.
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.