PCNA (Proliferating Cell Nuclear Antigen) is a 36kD protein also known as cyclin. In early S phase, PCNA has a very granular distribution and is absent from the nucleoli. At late S phase, PCNA is prominent in the nucleoli. There are two forms of PCNA protein present in cells, a soluble form sensitive to organic fixation and not involved in replication, and the other form is insoluble and is associated with ongoing DNA synthesis.
Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.
Western Blot: 0.5-4ug/ml
Immunohistochemistry: Frozen and paraffin
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.