The poly (ADP-ribose) polymerase (PARP) is involved in cell recovery from DNA damage, such as methylation of N3-adenine, which activates the base excision repair process. PARP [Poly (ADP-ribose) polymerase] is a 116kD nuclear chromatin-associated enzyme that is cleaved during apoptosis by caspase-3 into a 24kD fragment containing the DNA binding domain and an 89kD fragment containing the catalytic and automodification domains. The 24kD-fragment irreversibly bind to DNA and may contribute to the irreversibility of apoptosis by blocking the access of DNA repair enzymes to DNA strand breaks.
Suitable for use in Flow Cytometry and Western Blot. Other applications not tested.
Western Blot: 1-2ug/ml
Flow Cytometry: 1-3ug/test
Optimal dilutions to be determined by the researcher.
Camptothecin treated HL-60 (12 hr)
Storage and Stability:
May be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.